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Tissue typing is a procedure in which the tissues of a prospective donor and recipient are tested for compatibility prior to transplantation. An embryo can be tissue typed to ensure that the embryo implanted can be a cord-blood stem cell donor for a sick sibling.〔"Designing Donors". Shaun D. Pattinson, Faculty of Law and Sheffield Institute of Biotechnological Law and Ethics, University of Sheffield. (link )〕 One technique of tissue typing, "mixed leukocyte reaction", is performed by culturing lymphocytes from the donor together with those from the recipient.〔(MHC )〕 Another technique, known as a micro-cytotoxicity assay, utilizes serum with known anti-HLA antibodies that recognize particular HLA loci (HLA-A, HLA-B, HLA-C, HLA-DP, HLA-DQ, HLA-DR) in order to match genetically similar individuals in hopes of performing a tissue transplantation. In this technique a donor's blood cells are MHC typed by mixing them with serum containing the anti-HLA antibodies. If the antibodies recognize their epitope on the MHC then complement activation occurs and the cell will be osmotically lysed. Lysis results in the cell taking up a dye (trypan blue). This allows identification of cell's MHC indirectly based on the specificity of the known antibodies in the serum. ==See also== * Histocompatibility ==References== 〔 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「tissue typing」の詳細全文を読む スポンサード リンク
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